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Screensize: 640 x 480 800 x 600 1024 x 768 1280 x 1024 UserName: Password: Anti-Spam Code: Format Mode: Basic  Help  Prompt  Format: Font Andale Mono Arial Arial Black Book Antiqua Century Gothic Comic Sans MS Courier New Georgia Impact Lucida Console Script MT Bold Stencil Tahoma Times New Roman Trebuchet MS Verdana   Size 1 2 3 4 5 6   Color Black Red Yellow Pink Green Orange Purple Blue Beige Brown Teal Navy Maroon LimeGreen Message: * HTML is OFF * Forum Code is ON Smilies [quote][i]Originally posted by ivydong[/i] [br]Origin Ver. and Service Release (Select Help-->About Origin): 8.1 SR3 Operating System: Win 7 I need to process a huge number of fluorescence spectra, which have 4 peaks located at the exact same position but changes intensity with different spectra. The peak areas of all the 4 peaks are needed for further analysis. For every spectra, I need to subtract a same baseline and peak fit all the peaks to get the peak area(they are overlapped, so I can't just integrate areas.) I was trying to use peak analyzer and create a theme to do so, but couldn't get the baseline subtracted and find about 10 peaks instead. I really hope to get some help from this forum on how to conduct the process and if any language coding is needed, could someone give me some hint on how to do it? I'm so not familiar with software coding, please help. Thank you sooooooo much. :) [/quote]   Check here to include your profile signature. Check here to subscribe to this topic.