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Screensize: 640 x 480 800 x 600 1024 x 768 1280 x 1024 UserName: Password: Anti-Spam Code: Format Mode: Basic  Help  Prompt  Format: Font Andale Mono Arial Arial Black Book Antiqua Century Gothic Comic Sans MS Courier New Georgia Impact Lucida Console Script MT Bold Stencil Tahoma Times New Roman Trebuchet MS Verdana   Size 1 2 3 4 5 6   Color Black Red Yellow Pink Green Orange Purple Blue Beige Brown Teal Navy Maroon LimeGreen Message: * HTML is OFF * Forum Code is ON Smilies [quote][i]Originally posted by evamarianne[/i] [br]Origin Ver. and Service Release (Select Help-->About Origin): Operating System: I would need some help with the modification of the Hill Fit formula that I use for determining my Km and Vmax for MM kinetics. Since I have a protein showing product inhibition I would like to modify the formula including the inhibitory effect at higher substrate concentrations (the curve looks about like a skewed normal distribution). The formula I would like to use: y=Vmax*x^n/(k^n*(1+[I]/Ki))+x^n), where I would be the reversible inhibitor and Ki the rev. inhibitory constant. I already tried a lot of different things but I am not really sure whether it is possible to implement the end of the curve as 'inhibition effect' to determine the Km and Vmax for the full curve ... however I have seen full curve fits in other publications with horribly long formulas ... Thanks a lot in advance! [/quote]   Check here to include your profile signature. Check here to subscribe to this topic.