All Forums  Origin Forum  Origin Forum  obtaining broad baseline under sharp peaks

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Screensize: 640 x 480 800 x 600 1024 x 768 1280 x 1024 UserName: Password: Anti-Spam Code: Format Mode: Basic  Help  Prompt  Format: Font Andale Mono Arial Arial Black Book Antiqua Century Gothic Comic Sans MS Courier New Georgia Impact Lucida Console Script MT Bold Stencil Tahoma Times New Roman Trebuchet MS Verdana   Size 1 2 3 4 5 6   Color Black Red Yellow Pink Green Orange Purple Blue Beige Brown Teal Navy Maroon LimeGreen Message: * HTML is OFF * Forum Code is ON Smilies [quote][i]Originally posted by martin.ward[/i] [br]I have recently acquired fluorescence data that I wish to use to plot the emission data (the broad baseline). However, the data also shows sharp peaks attribute to another process (Raman scattering) that I wish to remove. Ideally I would be able to take my data set, find/fit the baseline. I have tried using the standard baseline finder in peak analyser; however, the output I obtain requires a lot of manual additions and deletions. Even after this the resultant baseline (fluorescence signal) is not very smooth and I find a significant variation of the peak emission value (peak of the broad baseline) after several repeat attempts. An example of the type of data I am working with can be found here (google drive): https://drive.google.com/open?id=0B4YTJ3V-IqLfamVaMmxuZ0xIWVk Any help/suggestions to resolve this problem would be greatly appreciated. Thanks! Version: OriginPro 2016. Service release: 2. Operating System: Windows 10 [/quote]   Check here to include your profile signature. Check here to subscribe to this topic.