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T O P I C    R E V I E W
Cat1987 Posted - 06/21/2017 : 06:40:46 AM
Origin Ver. and Service Release (Select Help-->About Origin):
Operating System:

Hi,

I am trying to plot a set of data for a KD using Hill's Model, however when I try to fit in the model, the Vmax given is almost double of the data points I have in the table and therefore the KD is 10 times higher than what it should be. I have processed a similar set of data without any problem so I am not sure what caused it? Could you help me please?

Many thanks in advance
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arstern Posted - 06/21/2017 : 3:42:14 PM
Hi,

Please check your parameters, most likely this is the cause of your large Vmax value. Have you tried changing the value of the Vmax parameter before selecting Fit?




Thanks,
Aviel
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