| T O P I C R E V I E W |
| quantusenor |
Posted - 04/05/2019 : 6:00:55 PM
Hello everyone. I am trying to fit my Kinetic Trace using the tutorial Fitting with convolution. I am total zero at coding, so when I try to write my own function, it doesn't work. The function I am using is tri-exponential-decay:
y=a1*exp(-(x-t0)/t1)+a2*exp(-(x-t0)/t2)+a3*exp(-(x-t0)/t3)
The purpose of whole fitting is to determine extinction coefficients of excited states, which can be found from pre-exponential coefficients.
I was thinking maybe I can deconvolute my trace and then fit in into Tri-exponential decay function?
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| 1 L A T E S T R E P L I E S (Newest First) |
| Sam Fang |
Posted - 04/08/2019 : 05:19:08 AM
Hi,
Origin provides Fit Convolution App for it, it needn't coding, and you can download and try it:
https://www.originlab.com/fileExchange/details.aspx?fid=423
The worksheet should include three columns like the tutorial: first column for x, second column for y (convoluted signal), and third column for impulse response (e.g. Gaussian).
Note that over-parameterization existed in your tri-exponential decay function. You can fix t0.
Sam
OriginLab Technical Services |
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