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Screensize: 640 x 480 800 x 600 1024 x 768 1280 x 1024 UserName: Password: Anti-Spam Code: Format Mode: Basic  Help  Prompt  Format: Font Andale Mono Arial Arial Black Book Antiqua Century Gothic Comic Sans MS Courier New Georgia Impact Lucida Console Script MT Bold Stencil Tahoma Times New Roman Trebuchet MS Verdana   Size 1 2 3 4 5 6   Color Black Red Yellow Pink Green Orange Purple Blue Beige Brown Teal Navy Maroon LimeGreen Message: * HTML is OFF * Forum Code is ON Smilies [quote][i]Originally posted by 73659[/i] [br]Origin Version (Select Help-->About Origin): Operating System: Windows XP The Analysis\Calculate\differentiate function can do differentiation. Unfortunately, if the neighboring data points contain error, the obtained derivative will enlarge the error, and contains too much noise. Sometimes the derivative may be too large (infinite, for example) or too small (0, for example) and its sign may be wrong. However, the general trend of my curve is very smooth and its derivative should be well-defined and definite. By trial-and-error method, I found the following method to solve the problem Use Analysis\Interpolate/Extrapolate to generate another set of data points that reflects the general smooth trend of the original curve; Differentiate the curve plotted using the 2nd set of data. My questions are (1) How to ensure the 2nd data set "resemble" the original data set? Specifically, how many data points are the best in the 2nd data set? (2) Is there any other, better method? [/quote]   Check here to include your profile signature. Check here to subscribe to this topic.