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Screensize: 640 x 480 800 x 600 1024 x 768 1280 x 1024 UserName: Password: Anti-Spam Code: Format Mode: Basic  Help  Prompt  Format: Font Andale Mono Arial Arial Black Book Antiqua Century Gothic Comic Sans MS Courier New Georgia Impact Lucida Console Script MT Bold Stencil Tahoma Times New Roman Trebuchet MS Verdana   Size 1 2 3 4 5 6   Color Black Red Yellow Pink Green Orange Purple Blue Beige Brown Teal Navy Maroon LimeGreen Message: * HTML is OFF * Forum Code is ON Smilies [quote][i]Originally posted by Jake Wimberger[/i] [br]Origin Ver. and Service Release (Select Help-->About Origin): trial Operating System:Win10 Has anyone had much experience with the peak deconvolution app? I'm trialing OriginPro for an application where peak deconvolution is necessary. I'm fitting data that has 7 populations/peaks, each with Gaussian distribution, none of them are baseline separated and many are just shoulders on other peaks. It's biological data so there's some noise. I've smoothed and constrained the peaks in a variety of ways, but continue to get fits that don't seem to follow the data very well even though the chi-squared says it's <1xE-6. As a comparison I fit it once manually and had it matching the data very well even though the chi-squared was over 200. Either something is wrong with how I'm using the deconvolution app, or the fitting algorithm is buggy. Any ideas? [/quote]   Check here to include your profile signature. Check here to subscribe to this topic.