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Screensize: 640 x 480 800 x 600 1024 x 768 1280 x 1024 UserName: Password: Anti-Spam Code: Format Mode: Basic  Help  Prompt  Format: Font Andale Mono Arial Arial Black Book Antiqua Century Gothic Comic Sans MS Courier New Georgia Impact Lucida Console Script MT Bold Stencil Tahoma Times New Roman Trebuchet MS Verdana   Size 1 2 3 4 5 6   Color Black Red Yellow Pink Green Orange Purple Blue Beige Brown Teal Navy Maroon LimeGreen Message: * HTML is OFF * Forum Code is ON Smilies [quote][i]Originally posted by Gonza984[/i] [br]Origin Ver. and Service Release (Select Help-->About Origin): Hi, I would like some help on the Fit Peaks feature of the peak analysis. I have 2,000 curves to analyze for peak detection, so I try to create a theme analysis. When I create the theme, in the Fit Peaks windows, Origin generates automatically some Gaussian extrapolation that fit perfectly with my curve (see picture attached where the green Gaussian fit my black curve in height). I checked "Generate report from current fitting result" and directly click on "Finish", without clicking on "Fit". The results are as I want for this curve. [img]https://my.originlab.com/ftp/forum_and_kbase/Images/Snap%20wanted.jpg[/img] Unfortunately when I save these parameters as a Theme and apply it automatically, the software seems to click on the "Fit" button and I obtain Gaussian that do not fit my curve height ans apparence (see picture attached). Do you know a way to reproduce exactly my first results with an automatic theme ? There is probably a box to check, but I may need help to find it. [img]https://my.originlab.com/ftp/forum_and_kbase/Images/Snap1%20unwanted.jpg[/img] Thanks all! [/quote]   Check here to include your profile signature. Check here to subscribe to this topic.