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Screensize: 640 x 480 800 x 600 1024 x 768 1280 x 1024 UserName: Password: Anti-Spam Code: Format Mode: Basic  Help  Prompt  Format: Font Andale Mono Arial Arial Black Book Antiqua Century Gothic Comic Sans MS Courier New Georgia Impact Lucida Console Script MT Bold Stencil Tahoma Times New Roman Trebuchet MS Verdana   Size 1 2 3 4 5 6   Color Black Red Yellow Pink Green Orange Purple Blue Beige Brown Teal Navy Maroon LimeGreen Message: * HTML is OFF * Forum Code is ON Smilies [quote][i]Originally posted by leejin[/i] [br]Greetings, i am quite new at this forum, as I am to Origin. Therefore I stand quite fast towards my first problem which even after some days of try I was unable to solve. The problem appears to be quite difficult to explain, despite I give my best ;) 1. We got a titration curve created with Origin for a photometric speciation. 2. According to the titration we got now several points within our coordinate system. After the application of the Boltzman-compensation we got a "clean" titration curve. 3. The next attemp is to make the evaluation by the tangents method. In this case according to the instructions we shall position parallel set tangents above and below of the inflection point The question is: how can the tangents being realized in Origin , including the in the image included normal ? with best regards Daniyal [/quote]   Check here to include your profile signature. Check here to subscribe to this topic.