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Screensize: 640 x 480 800 x 600 1024 x 768 1280 x 1024 UserName: Password: Anti-Spam Code: Format Mode: Basic  Help  Prompt  Format: Font Andale Mono Arial Arial Black Book Antiqua Century Gothic Comic Sans MS Courier New Georgia Impact Lucida Console Script MT Bold Stencil Tahoma Times New Roman Trebuchet MS Verdana   Size 1 2 3 4 5 6   Color Black Red Yellow Pink Green Orange Purple Blue Beige Brown Teal Navy Maroon LimeGreen Message: * HTML is OFF * Forum Code is ON Smilies [quote][i]Originally posted by jo137[/i] [br]Hi, does anybody know how I can make a 2-dimensional derivation in Origin 8? I have to analysize the fluorescence signal of a x-y-matrix (on x-axis there is the excitation wavelength, on y-axis there is the emission wavelength). How can I make the 2-dimensional derivation so I get as a result a 3-dimensional plot? Thanks for your help. Jo [/quote]   Check here to include your profile signature. Check here to subscribe to this topic.