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Screensize: 640 x 480 800 x 600 1024 x 768 1280 x 1024 UserName: Password: Anti-Spam Code: Format Mode: Basic  Help  Prompt  Format: Font Andale Mono Arial Arial Black Book Antiqua Century Gothic Comic Sans MS Courier New Georgia Impact Lucida Console Script MT Bold Stencil Tahoma Times New Roman Trebuchet MS Verdana   Size 1 2 3 4 5 6   Color Black Red Yellow Pink Green Orange Purple Blue Beige Brown Teal Navy Maroon LimeGreen Message: * HTML is OFF * Forum Code is ON Smilies [quote][i]Originally posted by najones84[/i] [br]Origin Ver. and Service Release (Select Help-->About Origin): Operating System: Hello, I was wondering if anyone can help me. I have a small data set of normalised values and the S.E.M, which i'm trying to plot a fitted exponential decay (first order). However, when i go to generate the fit an error message occurs saying "parameters are not properly initialized". This fitting worked fine on a same sized data set a few weeks ago and i just can't get my head around the problem (it's been a few days). I'm quite new to origin with no knowledge computer coding, so if anyone can give me a idiots guide to solve this problem it would be of much help. Below is the data set i'm trying to plot to get a nice graph, r2 value and the y= A1 *exp (-x/t1) + y0 equation Time Mean (normalised) SE 40 1 0 70 0.871997487 0.067707257 100 0.781273758 0.088397711 130 0.652044057 0.089901812 160 0.589689689 0.123395865 190 0.589514592 0.149950961 Thanks in advance Nick [/quote]   Check here to include your profile signature. Check here to subscribe to this topic.