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Fitting FRAP curves by Soumpasis Fit

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linktoaditya

Netherlands
1 Posts

Posted - 02/12/2015 : 11:35:44 AM

Hi,
I am looking for someone who is using the Soumpasis equation for fitting my data. Per se it is used for fitting one component fluorescent recovery curves.

I tried writing the code in Origin, but i am getting errors. I am not a programmer and hence do not understand these. Basically the equation is

where I0() is the modified Bessel function of the first kind of order 0 and I1() is the modified Bessel function of the first kind of first order to find only parameters A and #964;. A will be the mobile fraction and the diffusion coefficient,D is calculated by

D = w^2/Tau

The code i wrote in the code builder is :

//----------------------------------------------------------
//
void _nlsfSoumpasisFit(
// Fit Parameter(s):
double T,
// Independent Variable(s):
double y,
// Dependent Variable(s):
double& t)
{
// Beginning of editable part
exp^(-2*T/t)* {J0(2*T/t) + J1(2*T/t)}
// End of editable part
}

I might have made a blunder here. But i have no clue about programming as i am biologist. Any help would be highly appreciated!

Aditya

Aditya Iyer

jasonzhao

China
262 Posts

Posted - 02/16/2015 : 03:14:22 AM

Hello,

Please refer to this page for finer details.
http://www.originlab.com/doc/Tutorials/UserDef-FitFunc

you can write your fitting function in this way:


A*exp^(-2*T/t)*(J0*(2*T/t) + J1*(2*T/t))

the A, T, J0, J1 are parameters.

Best regards!
Jason
OriginLab Technical Service

Edited by - jasonzhao on 02/16/2015 03:59:58 AM

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