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 Association and Dissociation curve fitting		  New Topic  Reply to Topic
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sol708

USA
2 Posts
Posted - 08/07/2015 :  5:20:18 PM  Show Profile  Edit Topic  Reply with Quote  View user's IP address  Delete Topic
I am planning to create a user defined fitting function for the association and dissociation curves
To make a fitting of the first part of curve I have to use the following function
1 (Association):

y = Aeq * ( 1 - exp(- Kobs *t), where

t is independent parameter,
and Kobs, Aeq(Equivalent Signal) - parameters to be determined.

Once I get the values for these parameters , I have to use them for another fitting of the 2nd part of curve (function2 Dissociation):

y2 = Astop* exp( -Kdiss* ( t - Tstop ) );

where Astop = Aeq * ( 1 - exp(- Kobs *Tstop) ).

Is there any one can help me in creating a user-defined fitting function to get these Kd and Aeq values from function1 and use them automatically in the function2?
Thanks
Sol
Edited by - sol708 on 08/07/2015 7:18:29 PM

SeanMao

China
288 Posts
Posted - 08/11/2015 :  03:00:44 AM  Show Profile  Edit Reply  Reply with Quote  View user's IP address  Delete Reply
Hi,

You can create a piecewise function and directly use it to fit your data. Please refer to this page for step by step tutorial:
http://www.originlab.com/doc/Tutorials/Fitting-Piecewise

Alternatively, you can rearrange these two segments into different columns and global fit them with Kobs, Aeq as shared parameters:
http://www.originlab.com/doc/Tutorials/Multi-Functions-Global-Fitting

Regards!

Sean

OriginLab Tech Service
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