Could you please first check for updates to install onto your version of Origin. Select the Help menu and select Check Updates. I would also recommend to try repairing the Origin Installation by double-clicking the shortcut OriginPro Add or Remove files. This launches the Origin Setup files. Select Repair/Upgrade. Lastly try restarting your computer.
After taking a look at your OPJ file, it seems that you need to fix your axis for layer 2. I was able to determine this because in your OPJ file your graph shows that Layer 1 and Layer 2 both have their y-axis located on the left side. However you will want to setup the axis so that Layer 1 y-axis will be on the left side and Layer 2 y-axis will be on the right side. See image below to compare.
To set this up, first graph your A and B column as a column plot. With the graph activated right click the number 1 in the top left corner and select Layer Contents...
With the Layer Contents dialog box open select Layer(1) and Select New Layer. Here you will want to select Right-Y (Linked X scale and dimension). Right now I believe you have it setup as Left-Y (Linked X scale and dimension)
Next select Column C (Second option on left panel) and select the arrow button to transfer this selected data to Layer 2. Select Apply.