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 Subtracting fluorescence decay in Origin		  New Topic  Reply to Topic
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AnnLLLLLLLLLL

Finland
1 Posts
Posted - 11/07/2019 :  11:04:24 AM  Show Profile  Edit Topic  Reply with Quote  View user's IP address  Delete Topic
Hi all,

Can anyone recommend the easiest way to subtract fluorescence decay in Origin?

I have various datasets from GCAMP6m fluorescence measurements and need to subtract fluorescent decay from these graphs.

Thank you in advance,

Ann

AnnLLLLLLLLLL

lkb0221

China
497 Posts
Posted - 11/12/2019 :  3:33:23 PM  Show Profile  Edit Reply  Reply with Quote  View user's IP address  Delete Reply
Well... I'm afraid there would be no easy answer to that. You'll need to based on your actual case and define how the process would be. Like how much noise is there? Do they matter? How many decay stages are there? Do they overlapping or being high order decay? Etc.

You can try emailing Origin asking them to take a look.
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