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tibiriandre

Burkina Faso
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Posted - 01/16/2010 :  09:22:39 AM  Show Profile  Edit Topic  Reply with Quote  View user's IP address  Delete Topic
Origin Ver. and Service Release : OriginPro 8 SR6.

Hi
I want to perform One-Way ANOVA test with multiple comparaison.
I particularly want to compare data of many columns to a particular column that contains a standard data (Exple: IC50 of plant extracts versus IC50 of Quercetin).
How can I do this?

Thank for help !

larry_lan

China
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Posted - 01/17/2010 :  9:20:46 PM  Show Profile  Edit Reply  Reply with Quote  View user's IP address  Delete Reply
Hi:

ANOVA is comparing the mean of multiple DATASETs, while t-test can be used to compare the mean of ONE DATASET with A VALUE. Origin provide both of these functions under Statistics menu. When opening these dialogs, you can press F1 to open the help pages.

Could you give more detail requirement? I am not quite sure what do you exactly want, maybe you can contact us with your data through our support email.

Thanks
Larry
OriginLab Technical Services

Edited by - larry_lan on 01/18/2010 12:03:25 AM
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minimax

357 Posts

Posted - 01/17/2010 :  11:52:58 PM  Show Profile  Edit Reply  Reply with Quote  View user's IP address  Delete Reply
Hi Tibiri,

If your reference column is also a group of observations (not a single value), "Dunnett" is the usual method in multiple comparison to compare to a reference category, which is only available in Origin's Repeated Measures ANOVA, not in ordinary ANOVA yet.

Max
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tibiriandre

Burkina Faso
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Posted - 01/19/2010 :  05:13:36 AM  Show Profile  Edit Reply  Reply with Quote  View user's IP address  Delete Reply
Thank Larry, Minimax
Here is my problem.
I performed a experience with plant extracts with the aim to determine their antioxidant activity. I have 16 extracts of plant, let us say Extract1....Extract16. For each extract, the EC50 was determined in triplicate. I also performed the same experience with standards used as reference. Standards used are Quercetin, Rutin and Ascorbic acid. Now, I want to compare the antioxidant activity of my extracts to one of the standards.
I think I have to perform a One way ANOVA with a post test (the Dunnett post test). Since my measures are not repeated measures (I think well), I cannot. Is there another solution?
Thank many for help!
PS: DATA are been sent by email as recommanded by Larry.
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larry_lan

China
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Posted - 01/20/2010 :  05:47:20 AM  Show Profile  Edit Reply  Reply with Quote  View user's IP address  Delete Reply
Hi Tibiri:

Sorry that I didn't see your email, not sure what's wrong. Could you please send to tech support mail box and cc me the data again?

Thanks
Larry

Edited by - larry_lan on 01/20/2010 10:50:51 AM
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tibiriandre

Burkina Faso
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Posted - 01/20/2010 :  06:52:53 AM  Show Profile  Edit Reply  Reply with Quote  View user's IP address  Delete Reply
Hi Larry
Can you check your mail, I just sent the data.
Let me know whether you have received them.

Edited by - tibiriandre on 01/20/2010 07:05:46 AM
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larry_lan

China
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Posted - 01/20/2010 :  10:57:30 AM  Show Profile  Edit Reply  Reply with Quote  View user's IP address  Delete Reply
Yes, we got the data. We will look into that and reply.

Larry
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larry_lan

China
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Posted - 01/20/2010 :  10:42:04 PM  Show Profile  Edit Reply  Reply with Quote  View user's IP address  Delete Reply
Hi Tibiri:

Well, every group in your data have 3 values, including the standard group. I think the best method is Dunnett test. Sorry about that.

Larry
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tibiriandre

Burkina Faso
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Posted - 01/21/2010 :  06:30:24 AM  Show Profile  Edit Reply  Reply with Quote  View user's IP address  Delete Reply
Hi Larry
Thank for your reply. I'll try to get another tool.
Can I suggest that Origin developers include Dunnett test in One-Way ANOVA?
It would be very nice !
Thanks !
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