Origin 5.0 has a limit of 427 columns per worksheet. However, you can have multiple worksheets in the same project file so Origin can store all of your data in one project file...just not in one worksheet. Furthermore, you can plot data from different worksheets into the same graph window by adding the datasets via the Layer Contents dialog box (double click on the layer icon in the upper left corner of any graph window). This will allow you to plot any of your 1000 columns in one graph window.The Origin ASCII import routine has a column limit of 255 when importing ASCII files into worksheets (I am not an Excel expert by any means but it appears to me that Excel's import limit is 256). To work around this constraint, import your ASCII data into an Origin Matrix (Matrices have a much higher import routine column limit and a much higher column limit in general) and then copy paste the matrix columns into multiple worksheets. I was able to import over 1000 columns of data and plot them into one graph window using this method. Please note that there is also a limit of 427 datasets in one layer so you will need to create multiple layers in your graph window as well.