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 product inhibition - change of HillFormula		  New Topic  Reply to Topic
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evamarianne

Austria
1 Posts
Posted - 02/21/2014 :  11:18:08 AM  Show Profile  Edit Topic  Reply with Quote  View user's IP address  Delete Topic
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I would need some help with the modification of the Hill Fit formula that I use for determining my Km and Vmax for MM kinetics.

Since I have a protein showing product inhibition I would like to modify the formula including the inhibitory effect at higher substrate concentrations (the curve looks about like a skewed normal distribution). The formula I would like to use:
y=Vmax*x^n/(k^n*(1+[I]/Ki))+x^n), where I would be the reversible inhibitor and Ki the rev. inhibitory constant.
I already tried a lot of different things but I am not really sure whether it is possible to implement the end of the curve as 'inhibition effect' to determine the Km and Vmax for the full curve ... however I have seen full curve fits in other publications with horribly long formulas ...
Thanks a lot in advance!
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