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 obtaining broad baseline under sharp peaks
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martin.ward

United Kingdom
9 Posts

Posted - 01/30/2017 :  07:42:21 AM  Show Profile  Edit Topic  Reply with Quote  View user's IP address  Delete Topic
I have recently acquired fluorescence data that I wish to use to plot the emission data (the broad baseline). However, the data also shows sharp peaks attribute to another process (Raman scattering) that I wish to remove. Ideally I would be able to take my data set, find/fit the baseline.

I have tried using the standard baseline finder in peak analyser; however, the output I obtain requires a lot of manual additions and deletions. Even after this the resultant baseline (fluorescence signal) is not very smooth and I find a significant variation of the peak emission value (peak of the broad baseline) after several repeat attempts.

An example of the type of data I am working with can be found here (google drive): https://drive.google.com/open?id=0B4YTJ3V-IqLfamVaMmxuZ0xIWVk

Any help/suggestions to resolve this problem would be greatly appreciated.


Thanks!

Version: OriginPro 2016. Service release: 2. Operating System: Windows 10

Edited by - martin.ward on 01/30/2017 07:50:49 AM

Hideo Fujii

USA
1582 Posts

Posted - 01/30/2017 :  12:10:44 PM  Show Profile  Edit Reply  Reply with Quote  View user's IP address  Delete Reply
Hi martin.ward,

How about applying the median filter as in this document?
http://www.originlab.com/doc/Origin-Help/Smoothing

Hope this helps.

--Hideo Fujii
OriginLab
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