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 DNA Melting Curve (Tm) Fitting		  New Topic  Reply to Topic
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shoara

Canada
1 Posts
Posted - 06/01/2017 :  4:24:47 PM  Show Profile  Edit Topic  Reply with Quote  View user's IP address  Delete Topic
Origin Ver. and Service Release (Select Help-->About Origin):
Operating System: Win 7 Sp1, Origin 2016

Hi, I am trying to fit the normalized DNA melt curve data ( Absorbance vs. Temperature, C°). Do you know if there is any pre-defined fitting function available for that? If not, does anybody know how to define a sigmoidal melting curve function?

Thanks.



Aron Shoara

yuki_wu

896 Posts
Posted - 06/02/2017 :  01:19:08 AM  Show Profile  Edit Reply  Reply with Quote  View user's IP address  Delete Reply
Hi Aron,

Press F9 to open Fitting Function Organizer dialog, then expand Growth/Sigmoidal node, so you can check whether there is a built-in function for your case.

If not, you may need to build your own function. This tutorial can help:
http://www.originlab.com/doc/Origin-Help/UserDef-FitFunc

Regards,
Yuki
OriginLab
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