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kelly42
New Zealand
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 Posted - 10/08/2007 : 8:34:27 PM
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Origin Version (Select Help-->About Origin): 7.5 SR4 (7.5853)
Operating System: Windows XP SP2
I'm a new Origin user. I've been through the first eleven or so tutorials. My interest is in fitting sigmoidal data from an immunoassay using the five-parameter logistic (5PL) equation (similar to the built-in logistic, but with an extra parameter for asymmetry). After a couple of days of playing with the Advanced Fitting Tool, I think I finally have the curve fitting properly. I now have a curve relating measured absorbance (y) to the concentration of my molecule of interest (x).
This curve is only a small portion of my data. I want to now calculate the value of the independent variable (x) for a large set of absorbances (y values) using the curve I just fit. The Origin tutorials showed me how to do this in a point-by-point manner using the scripting window. I need to be able to batch several hundred data points through this process. This is a common and simple requirement for biochemists; how can I do it in Origin? (preferably without learning Origin C or scripting very much)
In the end, I need to be able to idiot-proof this process as much as possible to encourage my labmates to use Origin for their nonlinear curve fits instead of the Excel Solver (ick).
Thanks for your time!! |
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Calculating unknowns from curve fit
