I have a problem on conculating my data using the funtion of differentiate. If I want to select several data points to conculate the differentiate, what kind of funtion in origin I can use. By the way, what the formula of the differentiate funtion is.
If you are asking about numerical differentiation of a dataset, you can first create a graph of the x/y data, and then use the menu command Analysis->Calculus->Differentiate.
For details on how the differentiation is done, open the Origin help file (menu item: Help->Origin), go to Search tab, and type "differentiate" and look at the topic that comes up on top of list.
Note that differentiation can be done programmatically using LabTalk script or Origin C code as well. And if you need to use a particular algorithm of your own for differentiation, you can code that using script or Origin C.